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应用电化学法聚合酚藏花红(PPS)功能化的单壁碳纳米管,以其作为烟酰胺辅酶(NADH)氧化的电化学催化剂(电极),构建基于乙醇脱氢酶的安培型乙醇生物电化学传感器.该电极于0.0 V时,对NADH具有很好的催化性能.而单体酚藏花红则由于其电位过低(-0.48 V),不能显示催化性能.循环伏安和计时安培法测试表明:该传感器的碳纳米管的载量,固定化酶的量,NAD+的浓度以及溶液的pH都能直接影响它的性能.经优化制备的乙醇传感器在0.2 V电位下,对乙醇响应的灵敏度为2.0μA.cm-2.mM-1,检测限为36μmol.L-1.表现出很好的稳定性,连续测定45 min后,响应电流下降仅为起始值的7%.本文的研究为电化学生物传感器的创新开发提供了新的思路.
Electrochemical method was used to polymerize phenolic red phosphorus (PPS) functionalized single-walled carbon nanotubes as an electrochemical catalyst (electrode) for the oxidation of nicotinamide coenzyme (NADH) to construct an ethanol-based bioelectrode of ethanol dehydrogenase Chemical sensor.The electrode exhibited good catalytic performance for NADH at 0.0 V. However, the monomeric phenol red showed no catalytic activity due to its low potential (-0.48 V). Cyclic voltammetry and chronoamperometry The results showed that the capacity of carbon nanotubes, the amount of immobilized enzyme, the concentration of NAD + and the pH of the solution all have a direct effect on the performance of the sensor.The sensitivity of the ethanol sensor to ethanol response at 0.2 V Was 2.0μA.cm-2.mM-1, the detection limit was 36μmol.L-1.It showed good stability after 45min continuous measurement, the response current drop was only 7% of the initial value.This study It provides a new idea for the innovative development of electrochemical biosensors.