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植物在进化过程中针对干旱、高盐和高低温等逆境胁迫形成了多种适应机制,植物类受体激酶作为重要的细胞信号传递分子在植物生长和抗逆境胁迫中发挥着重要功能。该文发现一个具有S位点的类受体激酶基因OsSRL可能参与水稻(Oryzasativa)的干旱胁迫反应。利用RNAi技术降低OsSRL的表达水平后,转基因植株抗旱性增强,并表现出幼苗存活率、叶绿素含量及鲜重增加等表型。进一步的研究表明30%PEG和100μmol·L-1ABA可诱导OsSRL基因表达,利用RNAi降低其表达导致干旱诱导基因RAB16A及LEA3表达水平明显增加。表达模式分析发现OsSRL在胚芽、胚根、根、茎节以及花中表达。以上结果表明,OsSRL表达水平的降低增强植物的干旱耐受性,其作为一个S-位点样类受体激酶可能参与了水稻对干旱胁迫的反应。
Plants evolved a variety of adaptation mechanisms to drought stress such as drought, high salt and high and low temperature. Plant receptor kinase plays an important role in plant growth and stress-tolerance as an important cell signaling molecule. In this paper, we found that OsSRL, an S-site-like receptor kinase gene, may be involved in drought stress response in Oryza sativa. After RNAi was used to reduce the expression of OsSRL, the drought tolerance of transgenic plants was enhanced and the phenotypes of seedling survival, chlorophyll content and fresh weight increased. Further studies showed that 30% PEG and 100μmol·L-1ABA can induce OsSRL gene expression, RNAi reduce its expression led to drought-induced genes RAB16A and LEA3 expression increased significantly. Expression pattern analysis showed that OsSRL was expressed in germ, radicle, root, stem node and flower. The above results indicate that the decrease of OsSRL expression enhances the plant’s drought tolerance. As an S-site-like receptor kinase, it may be involved in the response of rice to drought stress.