建立了固相萃取-比色法测定西洋参为原料的保健食品中总皂苷含量的检测方法。通过前处理方法的对比优化及反应稳定性的相关性分析,找出最优方案。结果表明,在显色反应完成40 min内,该方法在0.015~0.152 mg范围内线性关系良好,r=0.999 6(n=6);在3个加标水平下平均回收率为98.45%,相对标准偏差为2.56%(n=9)。该方法简便、准确且稳定性、重复性好,回收率高,可用于以西洋参为代表的保健食品中总皂苷含量的测定和产品质量控制。 A method for the determination of total saponin in health foods using American ginseng as raw material was established by solid phase extraction and colorimetric method. Through the pretreatment method of comparison and analysis of the correlation between the stability of the reaction to find the optimal solution. The results showed that the linearity of the method was good in the range of 0.015 ~ 0.152 mg within 40 min after the completion of the chromogenic reaction, r = 0.999 6 (n = 6). The average recoveries at three spiked levels were 98.45% The standard deviation was 2.56% (n = 9). The method is simple, accurate and stable, has good repeatability and high recovery rate, and can be used for the determination of the total saponin content and the product quality control in the health foods represented by American ginseng.