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In our experiments the isolated rat adrenal glomerulosa cells displayed perlpheral-type benzodiazepine receptors, which could bind to [~3H] PKl1195 with an apparent equilibrium dissociation constant (KD) of 9.4±2.8 nmol/L and a maximal binding capacity (B_(max)) of 5.6q-1.8 pmol/10~6 cells. The effects of five ligands: PKl1195, Ro5--4846, flunitrazepam, diazepam and clonazepam on aldosterone secretion responses of isolated glomerulosa cells to anglotensin Ⅱ or extracellular potassium ions were observed. The logarithm of EO_(50) for these ligands as stimulators was well correlated with the logarithm of their Ki value for [~3H] PK11195 binding, suggesting that the stimulative effects might be mediated by the benzodiazepine receptor in isolated glomerulosa cells.
In our experiments of isolated rat adrenal glomerulosa cells displayed perlpheral-type benzodiazepine receptors, which could bind to [~ 3H] PK1195 with an apparent equilibrium dissociation constant (KD) of 9.4 ± 2.8 nmol / L and a maximal binding capacity (B_ (max ) of 5.6q-1.8 pmol / 10 ~ 6 cells. The effects of five ligands: PK1195, Ro5--4846, flunitrazepam, diazepam and clonazepam on aldosterone secretion responses of isolated glomerulosa cells to angiotensin II or extracellular potassium ions were observed. The logarithm of EO_ (50) for these ligands as stimulators was well correlated with the logarithm of their Ki value for [~ 3H] PK11195 binding, suggesting that the stimulative effects might be mediated by the benzodiazepine receptor in isolated glomerulosa cells.