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凤丹(Paeonia ostii)含有重要药用价值的次生代谢产物丹皮酚.丛枝菌根真菌(AM真菌)与凤丹存在密切的共生关系,菌根真菌能够直接或间接影响植物次级代谢过程.采用HPLC法测定凤丹中丹皮酚的含量、碱解离-酸性品红染色法测定侵染率、曲利苯兰染色法测定菌丝量、湿筛沉淀法测定孢子密度.结果发现,凤丹根际丛枝菌根侵染率、孢子密度和菌丝量与丹皮酚含量呈显著的正相关.本文通过AM真菌的孢子密度、菌丝量、侵染率与凤丹丹皮酚含量的相关性分析,表明AM真菌是凤丹中丹皮酚含量的促进因素,凤丹体内AM真菌侵染越强,根际土壤中AM孢子产生越多,土壤菌丝量越高,则丹皮酚含量积累越高.研究AM真菌对丹皮酚次级代谢物生成的影响,对进一步研究AM真菌对丹皮酚在药用价值方面提供重要依据.
Paeonia ostii contains paeonol, a secondary metabolite with important medicinal value. Arbuscular mycorrhizal fungi (Arbuscular mycorrhizal fungi) have a close symbiosis with Phoebe dandruff and mycorrhizal fungi can directly or indirectly affect plant secondary metabolites The content of paeonol was determined by HPLC, the rate of infection was determined by alkaline dissociation-acid fuchsin staining, the amount of mycelium was determined by transexiline staining, and the spore density was determined by wet sieving method. , There was a significant positive correlation between arbuscular mycorrhizal infection rate, spore density and mycelium content in the rhizosphere of Feng-dan.Among the AM fungi, spore density, mycelium amount, The correlation analysis of phenol content showed that AM fungi were the promoting factors of paeonol content in Fengdan. The stronger the AM fungi infection, the more AM spores in rhizosphere soil, the higher the amount of soil mycelium, The higher the accumulation of paeonol content.Studying the AM fungi paeonol secondary metabolites formation, the further study of AM fungi paeonol provide important basis for the medicinal value.