【摘 要】
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Human respiratory syncytial virus (RSV) is a major pathogen of acute lower respiratory tract infection among young children.To investigate the prevalence and genetic characteristics of RSV in China,we performed a molecular epi-demiological study during 20
【机 构】
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Beijing Key Laboratory of Pediatric Respiratory Infection Diseases,Key Laboratory of Major Diseases
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Human respiratory syncytial virus (RSV) is a major pathogen of acute lower respiratory tract infection among young children.To investigate the prevalence and genetic characteristics of RSV in China,we performed a molecular epi-demiological study during 2015-2019.A total of 964 RSV-positive specimens were identified from 5529 enrolled patients during a multi-center study.RSV subgroup A (RSV-A) was the predominant subgroup during this research period except in 2016.Totally,535 sequences of the second hypervariable region (HVR-2) of the G gene were obtained.Combined with 182 Chinese sequences from GenBank,phylogenetic trees showed that 521 RSV-A sequences fell in genotypes ON1 (512),NA1 (6) and GA5 (3),respectively;while 196 RSV-B sequences fell in BA9 (193) and SAB4 (3).ON1 and BA9 were the only genotypes after December 2015.Genotypes ON1 and BA9 can be separated into 10 and 7 lineages,respectively.The HVR-2 of genotype ON1 had six amino acid changes with a frequency more than 10％,while two substitutions H258Q and H266L were co-occurrences.The HVR-2 of genotype BA9 had nine amino acid substitutions with a frequency more than 10％,while the sequences with T290I and T312I were all from 2018 to 2019.One N-glycosylation site at 237 was identified among ON1 sequences,while two N-glycosylation sites (296 and 310) were identified in the 60-nucleotide duplication region of B A9.To conclusion,ON1 and B A9 were the predominant genotypes in China during 2015-2019.For the genotypes ON1 and BA9,the G gene exhibited relatively high diversity and evolved continuously.
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