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目的比较室温下改良HBSS液对体外培养人牙周膜细胞(PDLC)存活和克隆能力的影响。方法采用台盼蓝染色法和平板克隆形成试验检测体外培养的第4代人PDLC。在HBSS中添加青霉素、链霉素各100 U/ml、地塞米松8 mg/L及1、3、5 mmol/L的还原型谷胱甘肽(GSH)制成改良HBSS液,并与HBSS液比较,观察PDLC在上述溶液中保存不同时间后的细胞活性及克隆能力的变化。结果 3 mmol/L和5 mmol/LGSH组保存的细胞存活率在2~24 h显著高于HBSS液组,且5 mmol/LGSH组保存的PDLC克隆能力在1~12 h显著高于HBSS液组。结论含5 mmol/LGSH改良HBSS液保存人PDLC活性的效果在12 h内明显好于HBSS液。
Objective To compare the effects of modified HBSS solution on the survival and cloning ability of cultured human periodontal ligament cells (PDLC) in vitro. Methods The 4th generation human PDLC cultured in vitro was detected by trypan blue staining and plate clone formation assay. The HBSS solution was prepared by adding 100 U / ml penicillin, streptomycin, 8 mg / L dexamethasone and 1,3,5 mmol / L reducing glutathione (GSH) to HBSS, The cell viability and clonal abilities of PDLC after different time were observed in the above solution. Results The cell viability in 3 mmol / L and 5 mmol / L GSH groups was significantly higher than that in HBSS group from 2 to 24 h, and the cloning ability of PDLC in 5 mmol / L LGSH group was significantly higher than that in HBSS group from 1 to 12 h . Conclusion The PDLC activity of HBSS containing 5 mmol / L LGSH was significantly better than that of HBSS within 12 h.