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基于表达谱数据分析,根据公共数据库中的序列信息设计引物,通过RT-PCR扩增及克隆,从耐冷的东乡野生稻(Dongxiang Oryza rufipogon Griff.)中获得了在冷胁迫条件下高表达的热激转录因子BGIOSGA004402-DX的全长c DNA,与冷敏感水稻品种93-11的对应位点序列进行氨基酸同源比较分析,结果表明:该基因在2个不同材料中长度一样,都为249个氨基酸,具有99%的序列一致性,但存在2个氨基酸的差异,一个是第127号氨基酸,另一个是第155号氨基酸。
Based on the profiling data analysis, primers were designed based on sequence information in a public database, and high-expression heats were obtained from cold-tolerant Dongxiang Oryza rufipogon Griff. Under cold stress by RT-PCR amplification and cloning The results of amino acid homology analysis of the full-length c DNA of the transcription factor BGIOSGA004402-DX and the corresponding loci of the cold-sensitive rice variety 93-11 showed that the length of the gene was 249 in two different materials Amino acids, with 99% sequence identity, but with two amino acid differences, one is 127th amino acid and the other is 155th amino acid.