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目的评价市售某饮水机铜内胆中饮用水对大型溞的急性毒性效应,计算其半数有效浓度(EC50)和半数致死浓度(LC50)。方法以大型溞为模式生物,参照GB/T 16125—2012《大型溞急性毒性实验方法》,考察不同浓度的铜内胆饮用水对大型溞运动受抑制和死亡的影响,分析该饮水机铜内胆的化学元素组成以及试验水样中金属离子的浓度,探讨金属离子浓度与急性毒性效应之间的联系。结果该试验饮水机铜内胆中饮用水对大型溞24 h时EC50和LC50分别为5.55%和8.32%,48 h时EC50和LC50分别为3.58%和5.61%,铜内胆饮用水中铜和铅的浓度分别是GB 5749—2006《生活饮用水卫生标准》相应限值的10.51倍和1.17倍,铜内胆中饮用水的急性毒性效应随着大型溞暴露浓度的升高而增强。结论铜内胆中饮用水对大型溞有一定的急性毒性效应,使用时应注意健康防护。
Aim To evaluate the acute toxic effects of drinking water on the copper liner of a water dispenser on a commercial scale and calculate its half effective concentration (EC50) and half life lethal concentration (LC50). Methods According to GB / T 16125-2012 “Acute Toxicity Test Method for Large-scabies”, the effects of drinking water with different concentrations of copper liner on the inhibition and mortality of Sclarea scabies were investigated. The chemical composition of gallbladder and the concentration of metal ions in the test water sample to explore the relationship between the metal ion concentration and the acute toxicity effect. Results The drinking water in the copper liner of drinking fountains was 5.55% and 8.32% for 24 h, while the EC50 and LC50 were 3.58% and 5.61% at 48 h. The contents of copper and Lead concentrations were 10.51 times and 1.17 times the corresponding limits of the Standard for Drinking-water Quality of GB 5749-2006, respectively. The acute toxic effects of potable water in the copper liner were enhanced with the increase of the exposure concentration of M. macrorrhizae. Conclusion The drinking water in the copper liner has some acute toxic effects on the macrophus, and the health protection should be paid attention to when using it.