论文部分内容阅读
目的:观察DM120方对糖尿病大鼠肾组织氧化应激水平的影响。方法:高脂饲料联合链脲佐菌素(STZ)诱导糖尿病模型。随机分为正常对照组、模型组和DM120方治疗组,干预8周,观察各组大鼠血糖、肾功能及肾组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、8-异前列腺素F2α(8-iso-PGF2α)的变化。结果:模型组大鼠血糖、肌酐、尿素氮、24 h尿微量白蛋白排泄量水平均较正常对照组明显升高;DM120方治疗组大鼠血糖、肌酐、尿素氮及24 h尿微量白蛋白排泄量水平均较模型组下降(P<0.05,P<0.01);DM120方治疗组大鼠MDA、8-iso-PGF2α水平均较模型组下降(P<0.05),SOD水平较模型组上升(P<0.05),各组GSH-PX差异无统计学意义。结论:DM120方对糖尿病大鼠肾组织具有一定的抗氧化作用,其作用环节可能与MDA、SOD、8-iso-PGF2α相关。
Objective: To observe the effects of DM120 prescription on the oxidative stress in the kidney of diabetic rats. Methods: High-fat diet combined with streptozotocin (STZ) induced diabetic model. The rats were randomly divided into normal control group, model group and DM120 treatment group. After intervention for 8 weeks, blood glucose, renal function and MDA, SOD, Changes of peptide peroxidase (GSH-PX), 8-iso-prostaglandin F2α (8-iso-PGF2α). Results: The levels of blood glucose, creatinine, urea nitrogen and 24h urinary albumin excretion in model group were significantly higher than those in normal control group. The levels of blood glucose, creatinine, urea nitrogen and 24h urinary albumin (P <0.05, P <0.01). The levels of MDA and 8-iso-PGF2α in DM120 treatment group were significantly lower than those in model group (P <0.05) P <0.05). There was no significant difference in GSH-PX between groups. Conclusion: Diabetes mellitus (DM120) has certain anti-oxidative effects on the kidney of diabetic rats, and its action may be related to MDA, SOD and 8-iso-PGF2α.