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目的 :探讨大肠肿瘤P5 3mRNA转录改变。方法 :对 9例大肠癌和 3例良性大肠肿瘤石蜡标本的肿瘤灶、移行区和自身正常肠组织 ,应用反转录原位PCR技术进行了研究。结果 :3例良性肿瘤中仅 1例在肿瘤区组织检出弱阳性。 9例大肠癌中 ,肿瘤区检出 7例阳性 ,移行区检出 4例阳性 ,自身正常肠组织检出 1例阳性。结论 :在肿瘤早期即可能出现P5 3基因mRNA改变 ,应用RT CPRIS检出p5 3mRNA的阳性率高于常规免疫组化法。RT PCRIS具有很高的灵敏度和特异性 ,且可与组织结构联系进行分析
Objective: To investigate the transcriptional changes of P53 mRNA in colorectal tumors. METHODS: Retrospective RT-PCR was performed in 9 cases of colorectal cancer and 3 cases of benign colorectal tumors with paraffin-embedded tumor foci, transition zone and normal intestinal tissue. RESULTS: Only 3 cases of benign tumors detected weak positive tissue in the tumor area. In 9 cases of colorectal cancer, 7 cases were positive in the tumor area, 4 cases were positive in the transition area, and 1 case was positive in normal intestinal tissue. Conclusion : P5 3 mRNA may change in early stage of tumor. The positive rate of p53 mRNA detected by RT CPRIS is higher than that of routine immunohistochemistry. RT PCRIS has high sensitivity and specificity and can be linked to organizational structure for analysis