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采用反相高效液相色谱法 ,对膳食补充剂中的维生素C、叶酸、泛酸的同时测定进行了方法学研究。色谱参考条件为SupelcosilC18柱 ;柱温 :4 0℃ ;检测波长 :2 10nm ;流动相 :0 .0 5mol/LKH2 PO4∶CH3 OH∶0 .1mol/LKOH =85 +8+7(pH6 .3) ;流速 :1.5ml/min。用外标法定量 ,线性范围为维生素C :0～ 1.0mg/ml;叶酸 :0～ 0 .1mg/ml;泛酸 :0～ 0 .2 5mg/ml。线性相关系数为 0 .999到 0 .9997,平均回收率为 90 .5 %～ 10 5 .6 % ,相对标准偏差为 1.6 %～ 8.0 %。方法简便 ,快速、准确 ,用于实际样品测定 ,结果令人较为满意。
The method of simultaneous determination of vitamin C, folic acid and pantothenic acid in dietary supplements was studied by RP-HPLC. The chromatographic reference conditions were Supelcosil C18 column; the column temperature was 40 ° C; the detection wavelength was 210 nm; the mobile phase was 0.050 mol / L KH2 PO4:CH3 OH: 0.1 mol / L KOH = 85 +8 +7 (pH6.3) ; Flow rate: 1.5 ml / min. The external standard method with a linear range of vitamin C: 0 ~ 1.0mg / ml; folic acid: 0 ~ 0. 1mg / ml; pantothenic acid: 0 ~ 0. 25mg / ml. The linear correlation coefficients ranged from 0.999 to 0.9997. The average recoveries ranged from 90.5% to 105.6% with relative standard deviations from 1.6% to 8.0%. The method is simple, rapid and accurate and is suitable for the determination of actual samples. The result is satisfactory.