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高压电子射线处理过的人羊膜无抗原性,局部异物性刺激小,临床上取得了较好的效果。施术以陈旧性干穿孔(大小不拘)或不完全干的穿孔但至多一年中仅出现耳漏1～2次者为对象。术前行咽鼓管检查。未完全干耳者用抗生素治疗。人羊膜的制备:尽量无菌采集剖腹产的人羊膜,用生理盐水洗净,剪成2×2cm大小,并封入塑料袋中。浸放到干冰加99%乙醇内,移到电子加速器中,以每秒2.4×10~5r的比例,转8.4秒。总量200万r高压电子射线照射(150万V,15μA)之后,保存在-25℃的冷库内。需要时,用无菌生理盐水洗净,复温,放在无菌培养器上,把羊膜胎盘面,即有光泽的中胚叶面向上,展开,干燥后使用。鼓膜修补法:外耳道充分清拭,将浸湿鼓膜
High-voltage electron beam treatment of human amniotic membrane antigen-free, local stimulation of small foreign body, clinically achieved good results. The operation of the old dry perforation (size informal) or incomplete perforation but up to one year, only one or two times the occurrence of otic leaks. Preoperative Eustachian tube examination. Not completely dry ears were treated with antibiotics. Human amniotic membrane preparation: try to aseptically collected human amniotic membrane Caesarean section, washed with saline, cut into 2 × 2cm size, and sealed in plastic bags. Immersed in dry ice plus 99% ethanol, moved to the electron accelerator to 2.4 × 10 ~ 5r per second ratio, turn 8.4 seconds. The total amount of 2 million r high-voltage electron beam irradiation (1.5 million V, 15μA), stored in -25 ℃ cold storage. When necessary, wash with sterile saline, rewarming, on a sterile incubator, the amniotic placenta surface, that is, shiny mesodermal face up, expand, dry use. Tympanic membrane repair method: external auditory canal full cleaning, will wet the tympanic membrane