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通过HPLC技术测定槲皮苷含量并与其药材的傅里叶红外光谱进行相关分析,研究不同产地地枫皮槲皮苷含量相关的特征峰,试图快速筛选出高槲皮苷含量的优良地枫皮种质。结果显示:不同产地地枫皮间红外吸收光谱的峰形和峰位置均比较接近,但各谱峰的峰强有一定差异;为进一步研究与槲皮苷相关的吸收峰,对1732、1613、1518、1448、1373、1281、1158、1105、1056、824、771、663cm-1等处的峰强与实测槲皮苷含量的关系进行多元对数相关回归分析,结果显示:在1613、1448、1153、663cm-1等处附近的吸收强度与实际测定不同产地地枫皮槲皮苷含量均密切相关,R2=0.9800,P=0.002;两种方法均一致显示,槲皮苷含量以靖西化洞产地最高,其次是靖西安德和来宾忻城,其中以龙州产地含量最低。
Quercetin content was determined by HPLC and its correlation with the Fourier transform infrared spectroscopy of its medicinal materials to study the characteristic peaks related to quercetin content in different regions of origin, and the rapid screening of excellent Maple Germplasm The results showed that the peak shape and the peak position of infrared absorption spectra of the gastrodia abies in different areas were close, but the peak intensities of the peaks were different. To further study the absorption peaks related to quercitrin, 1518, 1448, 1373, 1281, 1158, 1105, 1056, 824, 771, 663 cm-1 and other peak intensity and quercetin content were multivariate logistic regression analysis showed that: at 1613,1448, 1153,663cm-1 and other near the absorption intensity and the actual determination of the origin of Quercus peel glucosides content are closely related, R2 = 0.9800, P = 0.002; two methods are consistent, quercitrin content to Jingxi Hidong The highest origin, followed by Jingdezhen Andre and guest Xincheng, of which the lowest content of Longzhou origin.