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四季桔成熟胚培养试管苗在普通培养室温度(25±2℃)下,在附加低浓度生长调节剂的大量元素减半的MS培养基上,每年继代1次或不需继代,大部分试管苗可连续保存2年,并且转移到新鲜培养基上可迅速恢复生长,大量增殖成苗。加入低浓度CCC和ABA等生长延缓剂可以适当降低生长速度。培养基失水干涸是延长继代时间的主要障碍之一
Four-year-old mature embryo culture in vitro test tube seedlings grown in ordinary culture room temperature (25 ± 2 ℃), in addition to the low concentration of growth regulator half the amount of MS medium, subculture once a year or without secondary, large Some of the test tube seedlings can be continuously stored for 2 years, and transferred to fresh medium can quickly resume growth, a large number of proliferation of seedlings. Addition of low concentrations of growth retardants such as CCA and ABA can reduce the growth rate. Drying medium is one of the main obstacles to extend the successor time