目的 :建立人单核巨噬细胞泡沫化抑制剂筛选模型 ,筛选得到可抑制细胞泡沫化的抑制剂。方法 :U937单核细胞经 10 0nmol·L-1佛波酯 (PMA)诱导 72h分化为巨噬细胞后 ,换无血清培养液于 96孔板中 ,每毫升含巨噬细胞 1× 10 6个 ,每孔再加入 80mg·L-1氧化的低密度脂蛋白 (ox -LDL) ,37℃培养 4 8h ,建立单核巨噬细胞泡沫化模型。利用微生物发酵液 ,或单一的化合物样品与其共孵育 ,油红。染色后观察细胞胞内变化 ,寻找对泡沫细胞形成有抑制作用的样品。利用基因工程技术表达的人清道夫受体A类II型的胞外部分 ,在本模型中可抑制巨噬细胞泡沫化的形成 ,进一步验证了模型的可行性。结果 :从 2 0 0 0个微生物发酵液中筛选到 6株微生物发酵液为阳性 ,从 10个化合物中发现一个有抑制巨噬细胞泡沫化活性的新化合物。结论 :本模型可用于细胞泡沫化抑制剂的高通量筛选 OBJECTIVE: To establish a screening model of human monocyte-macrophage foam inhibitor and screen for inhibitors that inhibit cell foam. METHODS: U937 monocytes were induced to differentiate into macrophages at 72 h after induction with 100 nmol·L-1 phorbol ester (PMA), and then replaced with serum-free medium in 96-well plates containing 1 × 10 6 macrophages Each well was then supplemented with 80 mg · L -1 oxidized low-density lipoprotein (ox-LDL) and incubated at 37 ° C for 48 h to establish a foam model of monocyte-macrophage. Use microbial fermentation broth, or a single sample of the compound incubated with it, oil red. After staining, the intracellular changes of the cells were observed to find samples that inhibited the foam cell formation. The extracellular part of human scavenger receptor A class II expressed by genetic engineering technology can inhibit the formation of macrophage foam in this model and further verify the feasibility of the model. Results: Six strains of microorganisms were screened from 200 strains of microorganism fermentation broth, and 10 compounds were found to have a new compound which could inhibit the foam activity of macrophages. Conclusion: This model can be used for high-throughput screening of cell foam inhibitors