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用灭活的〔Flammulinasubnudus (Pr .)Sing〕双核菌株原生质体与〔Flammulinavelutipes (Fr.)Sing〕双核菌株原生质体融合 ,在 2 2℃的条件下筛选得融合子。融合频率为 0× 10 -5~ 4 1× 10 -5。融合子与双亲有明显的拮抗性。融合子的菌丝体形态 ,氨基酸含量 ,子实体的形态 ,以及酸性磷酸酶的测定都与双亲不同。
Fusion of the inactivated strain of Flammulinasubnudus (Pr.) Sing] protoplasts with protoplasts of Flammulinavelutipes (Fr.) Sing was performed and the fusion was screened at 22 ° C. The fusion frequency is 0 × 10 -5 ~ 4 1 × 10 -5. Fusion and parents have obvious antagonism. The mycelium morphology, amino acid content, fruiting body morphology, and acid phosphatase assay of fusion were different from those of parents.