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①目的观察牛磺酸对小鼠脾细胞DNA修复能力的影响,以探讨牛磺酸抗衰老的机制。②方法以不同鼠龄小白鼠脾细胞悬液进行培养,实验组培养液中加入牛磺酸(30μg/ml),以10mmol/L羟基脲抑制DNA复制,经紫外线照射后,以3H-胸腺嘧啶脱氧核苷(3H-TdR)掺入法测定DNA损伤后修复能力。③结果鼠龄110d,150d和180d3组小鼠中,DNA修复能力均随鼠龄增加而降低,但在不同鼠龄阶段,牛磺酸组DNA修复能力均高于对照组,牛磺酸组的闪烁值分别为8.0±0.6Hz,5.3±0.5Hz和4.1±0.4Hz,而对照组则分别为6.3±0.7Hz,4.7±0.5Hz和3.2±0.2Hz(t=7.50,2.87,33.80,P均<0.01)。④结论牛磺酸具有增强小鼠脾细胞DNA的修复能力和延缓衰老的作用。
Objective To observe the effect of taurine on the DNA repair ability of mouse spleen cells to explore the mechanism of taurine anti-aging. Methods The spleen cell suspension of mice of different age was cultured. Taurine (30μg / ml) was added into the culture medium of experimental mice to inhibit DNA replication with 10mmol / L hydroxyurea. After UV irradiation, 3H-thymine Deoxyribonucleoside (3H-TdR) incorporation assay for DNA repair capacity after injury. ③Results In the mice of 110d, 150d and 180d3 groups, DNA repair ability decreased with the increase of the age of the mice, but the DNA repair capacity of the taurine group was higher than that of the control group at different stages The flicker values were 8.0 ± 0.6Hz, 5.3 ± 0.5Hz and 4.1 ± 0.4Hz respectively, while those of the control group were 6.3 ± 0.7Hz, 4.7 ± 0.5Hz and 3.2 ± 0.2Hz (t = 7.50,2.87,33.80, P <0.01). ④ Conclusion Taurine can enhance DNA repair ability of mice spleen cells and anti-aging effect.