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目的:探讨胃宁颗粒对人胃癌裸鼠原位移植瘤生长及转移的抑制作用。方法:制作人胃癌裸鼠原位移植模型,随机分成对照组、环磷酰胺(CTX)组、胃宁颗粒组、CTX+胃宁颗粒组,每组12只,观察瘤体积抑制率、瘤重量抑制率、肝转移发生率、肿瘤组织细胞形态学变化、凋亡指数(AI)、p53、Bcl-2、bax、CD44v6、增殖细胞核抗原(PCNA)蛋白表达的变化。结果:CTX组、胃宁颗粒组、胃宁颗粒组+CTX组和对照组相比,瘤重抑制率、肿瘤体积抑制率差异有显著性(P<0.05);胃宁颗粒组+CTX组与CTX组相比,瘤重抑制率、肿瘤体积抑制率差异有显著性(P<0.05);胃宁颗粒组、CTX组肝转移发生率与对照组相比,差异有统计学意义(P<0.05或P<0.01);胃宁颗粒组与对照组AI值相比,差异有显著性(P<0.01);胃宁颗粒组p53、bcl-2、bax、CD44v6、PCNA与对照组相比,差异有统计学意义(P<0.05);胃宁颗粒+CTX组与CTX组相比,p53、CD44v6、PCNA表达差异有显著性(P<0.05)。结论:胃宁颗粒具有抑制人胃癌裸鼠原位移植瘤生长及转移作用。
Objective: To investigate the inhibitory effect of Weining granule on the growth and metastasis of human gastric cancer xenografts in nude mice. METHODS: The orthotopic transplantation model of gastric cancer in nude mice was established and randomly divided into control group, cyclophosphamide (CTX) group, Weining Granule group and CTX + Weining Granule group, with 12 rats in each group. The tumor volume inhibition rate and tumor weight inhibition The incidence of hepatic metastasis, tumor cell morphological changes, apoptosis index (AI), p53, Bcl-2, bax, CD44v6 and proliferating cell nuclear antigen (PCNA) Results: Compared with the control group, the tumor weight inhibition rate and the tumor volume inhibition rate of CTX group, Weining Granule group and Weining Granule group + CTX group were significantly different (P <0.05) (P <0.05). The incidence of hepatic metastasis in Weining granule group and CTX group was significantly lower than that in control group (P <0.05) Or P <0.01). There was significant difference in the AI value between Weining granule group and control group (P <0.01). The difference of p53, bcl-2, bax, CD44v6, (P <0.05). The expression of p53, CD44v6 and PCNA in Weining granule + CTX group was significantly higher than that in CTX group (P <0.05). Conclusion: Weining Granule can inhibit the growth and metastasis of orthotopic transplanted human gastric cancer in nude mice.