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目的:建立肠肽胶囊中三七有效成分的HPLC含量测定方法。方法:色谱柱为SinoChrom ODS-BP(150 mm×4.6mm,5μm),以乙腈(A)和水(B)为流动相,梯度洗脱,检测波长为203 nm,流速为1.0 ml·min~(-1),柱温为室温,进样量为20μl。结果:三七皂苷R_1、人参皂苷Rg_1及人参皂苷Rb_1的线性范围依次为63.9～319.5μg·ml~(-1)、112.6～563.2μg·ml~(-1)、102.5～512.8μg·ml~(-1),线性关系良好(r值分别为0.999 7、0.999 4、0.999 9);三种皂苷的平均回收率分别为96.47%、98.75%,97.55%,RSD分别为1.52%、1.73%、1.81%(n=6)。结论:本方法简便可行、准确、灵敏度高、专属性强,可用于肠肽胶囊的质量控制。
Objective: To establish a HPLC method for the determination of effective components in Panax notoginseng capsule. METHODS: The chromatographic column was SinoChrom ODS-BP (150 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile (A) and water (B) with a gradient of 203 nm and a flow rate of 1.0 ml · min ~ (-1). The column temperature was room temperature and the injection volume was 20μl. Results: The linear ranges of notoginsenoside R 1, ginsenoside Rg 1 and ginsenoside Rb 1 were 63.9 ~ 319.5μg · ml -1, 112.6 ~ 563.2μg · ml -1, 102.5 ~ 512.8μg · ml -1, (-1) and good linearity (r = 0.999 7,0.999 4,0.999 9, respectively). The average recoveries of the three saponins were 96.47%, 98.75% and 97.55%, respectively, with RSDs of 1.52% and 1.73% 1.81% (n = 6). Conclusion: The method is simple, feasible, accurate, sensitive and specific. It can be used for the quality control of gut peptide.