代表性差异分析法(RDA,representa-tional difference analysis)是近年发展的一种经改进的扣除杂交法(subtractive hy-bridization)。 作者用酚/氯仿分别从50条雌或雄成虫中抽提基因组DNA,按Lisitsyn等方法进行代表性差异分析。以HindⅢ消化DNA后与R Hind 24(5′—3′)和R Hind 12(5′—3′)接头连接,用R Hind 24作引物进行PCR。雌虫 Representational difference analysis (RDA) is an improved subtractive hybridization (subtractive hy-bridization) developed in recent years. The authors extracted genomic DNA from 50 female or male adults using phenol / chloroform, respectively, and performed representative differences analysis by Lisitsyn et al. The DNA was digested with HindIII and ligated with the R Hind 24 (5’-3 ’) and R Hind 12 (5’-3’) linkers, and PCR was performed using R Hind 24 as a primer. Female