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目的:探讨温郁金醚提物中二萜类化合物C体外对人结肠腺癌SW620细胞的抑制作用及机制。方法:将不同浓度的化合物C作用SW620细胞后,采用MTT法检测细胞的增殖情况,倒置相差显微镜下观察细胞的形态学变化,流式细胞术检测细胞的周期分布和凋亡率。结果:化合物C能以时间-浓度依赖性方式抑制SW620细胞增殖,其抑制能力显著高于阳性对照药5-Fu和L-OHP(P<0.01),而对正常HUVEC细胞仅有轻微毒性作用;作用后的细胞在镜下呈典型的凋亡改变;化合物C能阻滞细胞于G1和S期,减少G2期比例,并以时间-浓度依赖性方式显著诱导细胞凋亡(P<0.01)。结论:温郁金中二萜类化合物C能显著抑制SW620细胞的增殖,并阻滞细胞周期和诱导细胞凋亡,这可能是其发挥抗肿瘤作用的机制之一。
OBJECTIVE: To investigate the inhibitory effect and mechanism of diterpene C on human colon adenocarcinoma SW620 cells in vitro. Methods: The proliferation of SW620 cells treated with different concentrations of compound C was detected by MTT assay. The morphological changes of cells were observed under inverted phase contrast microscope. The cell cycle distribution and apoptosis rate were detected by flow cytometry. Results: Compound C could inhibit the proliferation of SW620 cells in a time-and concentration-dependent manner, and its inhibitory ability was significantly higher than that of the positive control drugs 5-Fu and L-OHP (P <0.01), but only slight toxicity to normal HUVEC cells. After treatment, the cells showed typical apoptotic changes in the microscope. Compound C could block cells in G1 and S phase and decrease the proportion of G2 phase, and significantly induced apoptosis in a concentration-dependent manner (P <0.01). CONCLUSION: Diterpenoid C in Wenyujin can significantly inhibit the proliferation of SW620 cells and arrest the cell cycle and induce apoptosis, which may be one of the mechanisms by which diterpenoid C exerts its anti-tumor effect.