论文部分内容阅读
为了合理评价北方地区白菜型油菜种质资源,为抗寒育种及组合配制提供依据,从252对标记中筛选出9对SSR和36对In Del标记对感温性、抗寒性、品质等性状差异明显的19份白菜型油菜品种的遗传多样性和亲缘关系进行了分析。结果表明,共检测到95个等位变异,平均每个标记2.15个;有效等位基因变幅为1.05~3.27个,平均为1.70个。Shannon指数的变幅范围在0.121 7~1.269 5间,平均值为0.580 3;多态性信息量PIC变幅范围为0.049 9~0.637 7,平均值为0.308 1。通过NTSYS计算遗传相似系数GS并按加权配对法(UPGMA)聚类,结果表明19份材料的GS变异在0.52~0.86之间。在GS为0.605水平上可将19份材料按两室与多室性划分为I-1和I-2两大类群,I-1类群在GS为0.655水平上可按冬春性分为II-1、II-2两个亚类群。在GS为0.71水平上,可将冬性材料划分为4个小群。聚类结果表明北方地区白菜型油菜的遗传多样性丰富,冬、春性品种间及春性品种间亲缘关系较远,存在较大遗传差异。
In order to reasonably appraise the germplasm resources of Brassica campestris in the northern area and provide the basis for cold breeding and combination preparation, nine pairs of SSR markers and 36 pairs of In Del markers were selected for the evaluation of temperature resistance, cold resistance and quality The genetic diversity and genetic relationship of 19 Chinese cabbage rape cultivars with obvious differences were analyzed. The results showed that a total of 95 alleles were detected with an average of 2.15 for each marker. The effective allele amplitude ranged from 1.05 to 3.27 with an average of 1.70. The range of Shannon index ranged from 0.121 7 to 1.269 5 with an average of 0.580 3. The range of polymorphic information PIC ranged from 0.049 9 to 0.637 7 with an average of 0.308 1. The genetic similarity coefficient GS was calculated by NTSYS and clustered according to the weighted matching method (UPGMA). The results showed that the GS variation of 19 cultivars ranged from 0.52 to 0.86. Nineteen materials could be divided into two major groups I-1 and I-2 according to two-compartment and multi-compartment GS at the level of GS of 0.605. The population of I-1 was divided into II- 1, II-2 two subgroups. At a GS level of 0.71, winter material can be divided into four subgroups. The clustering results showed that the genetic diversity of Brassica napus in northern China is rich. The genetic relationship between winter and spring varieties and spring varieties is far, and there is a big genetic difference.