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目的 探讨维拉帕米能否提高神经母细胞瘤细胞对长春新碱的敏感性。方法 对 2 3例神经母细胞瘤组织进行分离获得肿瘤细胞 ,采用原代细胞培养及MTT检测药敏的方法 ,测定了长春新碱及其与维拉帕米合用时对肿瘤细胞的抑制率。结果 未经化疗的患者 ,合用维拉帕米后不能增加肿瘤细胞对长春新碱的敏感性 (P >0 .0 5 ) ;化疗过的患者 ,长春新碱与维拉帕米合用时 ,对肿瘤细胞的抑制率明显高于单用长春新碱者 (P <0 .0 5 )。结论 化疗后对长春新碱敏感性降低的神经母细胞瘤患者 ,化疗时加用维拉帕米可望提高化疗效果。
Objective To investigate whether verapamil can increase the sensitivity of neuroblastoma cells to vincristine. Methods The tumor cells were isolated from 23 neuroblastoma tissues and the inhibitory rate of vincristine and verapamil on the tumor cells was determined by primary cell culture and MTT assay. The results of patients without chemotherapy, combined with verapamil can not increase the sensitivity of tumor cells to vincristine (P gt; 0 5); chemotherapy patients, vincristine and verapamil combined The inhibition rate of tumor cells was significantly higher than that of vincristine alone (P <0.05). Conclusion Chemotherapy after vincristine sensitivity of patients with neuroblastoma chemotherapy plus verapamil is expected to improve the effect of chemotherapy.