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由于在水体生态环境系统中的重要功能及化学组成和结构的复杂性,天然溶解有机质(DissolvedOrganicMatter,DOM)是近年来水环境研究领域的热点和难点问题之一.分子荧光特征和分子量分布被广泛认为是DOM特征研究的重要参数,但是它们之间的关系尚不清楚.本文联合运用分子荧光光谱,高效液相体积排阻色谱、紫外吸收和荧光在线检测技术,研究了贵州红枫湖和百花湖及其河水中溶解有机质的分子荧光与分子量分布特征,及它们之间的关系.水体中DOM普遍存在两个明显的类腐殖荧光峰(A和B),但河水还存在另一个特征明显的类蛋白荧光峰(C).研究结果表明:类腐殖荧光物质主要由1.0~3.0kD之间的小分子组分组成,而类蛋白荧光物质主要是一些分子量大于2.0kD的组分组成.运用高效液相体积排阻色谱技术测定DOM分子量分布时,估算的分子量分布特征与吸收光谱检测器的波长选择有关.
Dissolved Organic Matter (DOM) is one of the hot and difficult problems in the field of water environment research in recent years because of its important functions and chemical composition and structure complexity in water ecological environment system.Molecular fluorescence characteristics and molecular weight distribution are widely Which is regarded as an important parameter for the study of DOM characteristics.However, the relationship between them is not yet clear.In this paper, molecular fluorescence spectrometry, high performance liquid chromatography (HPLC), ultraviolet absorption and fluorescence on-line detection were used to study the relationship between Hongfeng Lake and Baihua Fluorescence and molecular weight distribution of dissolved organic matter in lakes and their rivers, and the relationship between them. Two obvious humus-like fluorescence peaks (A and B) exist in the DOM, but another obvious characteristic (C) .The results show that the humus-like fluorescent substance mainly consists of small molecular components between 1.0 and 3.0 kD, whereas the protein-like fluorescent substances are mainly composed of components with a molecular weight greater than 2.0 kD. Estimation of molecular weight distribution and wavelength selection of absorption spectroscopy detector when determining DOM molecular weight distribution by high performance liquid chromatography-exclusion chromatography turn off.