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我国具有丰富的地方品种,但在大豆育种中利用率较低,这与对地方品种的保存和鉴定研究较少有关。通过利用全基因组SSR标记扫描的方法对地方品种羊眼豆和毛豆进行纯度鉴定,旨在为地方品种的纯化及遗传完整性的保存提供科学依据。结果表明:羊眼豆在502个SSR标记位点的多态性为44.8%,毛豆在405个SSR标记位点的多态性为41.2%;检测2个地方品种纯度所需的最少标记数和种子粒数分别为3个标记和4粒种子;根据遗传一致性分别对2个地方品种进行聚类,在相似系数为0.85时,均被分为3个组。因此,这2个地方品种纯度较低,根据遗传一致性分组情况,对两个地方品种的一组进行提纯后作为一个品种保存,而另外2个组则可分别作为2个不同的地方品种保存其遗传完整性。
China has rich local varieties, but its utilization rate in soybean breeding is low, which is less related to the research on the preservation and identification of local varieties. The purity of local varieties of Eyed Peas and Glycine soja was characterized by using genome-wide SSR marker scanning, which provided a scientific basis for the purification of local breeds and the preservation of genetic integrity. The results showed that the polymorphism of sheep eyes was 44.8% at 502 SSR markers and that of soybean at 405 SSR markers was 41.2%. The minimum number of markers needed to detect the purity of two local breeds Seed numbers were 3 and 4 seeds, respectively. According to the genetic identity, 2 landraces were clustered respectively. When the similarity coefficient was 0.85, they were divided into 3 groups. Therefore, the purity of these two local breeds was low, one of the two local breeds was purified as a breed according to the genetic grouping, while the other two breeds could be stored separately as two different breeds Its genetic integrity.