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血清是组织和细胞培养基的主要组成之一,它起着各种作用,而促进细胞分裂是其主要作用。虽胎牛血清、鸡血清、人AB血清及其他动物血清均可应用,但都因来源困难或价格昂贵而受限,以胎牛血清应用最广。但近年商品胎牛血清供应紧张,且质量不够稳定,对开展细胞培养工作带来一定影响。我们在前人应用人脐血清作培养基成份的基础上,利用本院有利条件进行了实验,于一九八○年开始试用本院产房采集的脐血混合血清作外周血(50例)和羊水细胞(20例)培养,其结果完全可代替胎牛血清。
Serum is one of the major components of tissue and cell culture media. It plays a variety of roles, and promoting cell division is its primary role. Although fetal bovine serum, chicken serum, human AB serum and other animal serum can be applied, but because of the source is difficult or expensive and limited, the most widely used fetal bovine serum. In recent years, however, the supply of commercial fetal bovine serum has been in short supply and the quality is not stable enough, which has brought a certain impact on the work of cell culture. On the basis of our predecessors’ application of human umbilical cord blood serum as the culture medium, we conducted experiments using the favorable conditions in our hospital. From 1980, we started to test peripheral blood (50 cases) of umbilical cord blood mixed serum collected from the delivery room of our hospital and Amniotic fluid cells (20 cases) culture, the results can replace fetal bovine serum.