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目的：制备抗人甲状腺球蛋白（Ｔｇ）单克隆抗体，为建立高灵敏度的Ｔｇ检测方法做准备。方法：从人的甲状腺组织中提取Ｔｇ做抗原，经腹腔免疫ＢＡＬＢ／ｃ小鼠，取其脾细胞与小鼠骨髓瘤细胞系ＳＰ２／ｏ融合，筛选及克隆化。结果：获得２株分泌抗人Ｔｇ单克隆抗体的杂交瘤细胞株。鼠腹水抗体效价达１．０２×１０６。经免疫球蛋白类别及亚类的鉴定，２株单克隆抗体均为ＩｇＧ１亚类，轻链为Ｋ型。结论：我们制备的抗Ｔｇ单克隆抗体具有良好的免疫反应特性，符合Ｔｇ酶联免疫分析法（ＥＬＩＳＡ）的要求
OBJECTIVE: To prepare anti-human thyroglobulin (Tg) monoclonal antibody in preparation for the establishment of high sensitivity Tg detection method. Methods: Tg was extracted from human thyroid tissue and BALB / c mice were immunized intraperitoneally. The spleen cells were fused with mouse myeloma cell line SP2 / o and screened and cloned. Results: Two hybridoma cell lines secreting anti-human Tg monoclonal antibody were obtained. Murine ascites antibody titer of 1.02 × 106. The immunoglobulin class and subclass identification, two monoclonal antibodies are IgG1 subclasses, the light chain is K-type. CONCLUSION: The anti-Tg monoclonal antibodies we prepared have good immunoreactivity and meet the requirements of the Tg enzyme-linked immunosorbent assay (ELISA)