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目的:探讨十二味穿甲片对高脂性大鼠脂肪肝模型的防治作用。方法:将SD鼠随机分为空白对照组、模型对照组、十二味穿甲片原料2.8,1.4,0.7 g·kg~(-1)剂量组和阳性对照组。除空白对照组灌胃(ig)生理盐水外,其余各组大鼠灌胃脂肪乳剂,每日早上9:00给予1次、连续5周,建立高脂性脂肪肝大鼠模型。造模同时灌胃给药(供试药采用十二味穿甲片的原料浸膏粉),每日下午16:00给予1次,5周后称取体质量、肝湿重,计算肝指数,检测大鼠血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、甘油三脂(TG)、总胆固醇(TC)含量,镜下观察各组大鼠肝组织形态学变化。结果:与空白对照组比较,模型对照组大鼠血清TC、TG和肝湿重显著增加,肝指数上升,差异具有统计学意义(P<0.05或0.01);血清ALT、AST明显升高,但无统计学意义(P>0.05);镜下观肝小叶细胞内弥漫性密集细小的脂滴空泡。与模型对照组比较,十二味穿甲片原料2.8,1.4,0.7 g·kg~(-1)剂量组肝湿重、肝指数,以及血清TC、TG、ALT均有下降,其中十二味穿甲片原料0.7 g·kg~(-1)剂量组肝指数差异显著(P<0.05)、十二味穿甲片原料各剂量组血清TC,以及1.4,0.7 g·kg~(-1)剂量组血清TG差异显著(P<0.05或0.01);镜下观十二味穿甲片原料2.8,1.4 g·kg~(-1)剂量组大鼠肝细胞索排列整齐,肝窦清晰可见,细胞质内脂滴消失,肝细胞结构正常,较模型对照组显著改善,十二味穿甲片原料0.7 g·kg~(-1)剂量组肝脂肪变性与模型对照组比无明显改善。结论:十二味穿甲片原料具有良好的抗高脂性脂肪肝作用,并呈现一定的量效关系。
Objective: To investigate the preventive and therapeutic effects of 12-flavorant on the fatty liver model of hyperlipidemic rats. Methods: SD rats were randomly divided into blank control group, model control group, 12, 8, and 10 μg · kg -1 dosage groups and the positive control group. In addition to the blank control group intragastric (ig) saline, the remaining groups of rats fed with fat emulsion, given once daily 9:00 am for 5 consecutive weeks to establish a model of fatty liver in rats. At the same time, the model was given intragastric administration (for the reagent, the raw material extract powder of twelve-flavored puncture-piercing tablets) was given once a day at 16:00 pm. After 5 weeks, the body weight and liver wet weight were weighed and the liver index The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG) and total cholesterol (TC) were measured. The morphological changes of liver tissue were observed under microscope. Results: Compared with the blank control group, the serum TC, TG and liver wet weight of the model control group increased significantly and the liver index increased, the difference was statistically significant (P <0.05 or 0.01); the serum ALT and AST were significantly increased No statistical significance (P> 0.05); microscopic observation of hepatic lobule cells diffuse dense small droplets of lipid vacuoles. Compared with the model control group, the values of liver wet weight, liver index, serum TC, TG and ALT in the 12, 8 and 10 g · kg -1 groups were all decreased The difference of liver index between the two groups was statistically significant (P <0.05), the TC of each dose of 12 tablets and the dosage of 1.4, 0.7 g · kg ~ (-1) (P <0.05 or 0.01). Under the microscope, the hepatic cord of rats in the dose of 2.8 and 1.4 g · kg ~ (-1) The lipid droplets in the cytoplasm disappeared and the hepatocyte structure was normal. Compared with the model control group, the lipid droplets in the cytoplasm disappeared and the hepatic steatosis in the dose of 0.7 g · kg -1 was significantly improved compared with the model control group. Conclusion: Twelve wearable tablets have good anti-fatty liver effect and show a certain dose-effect relationship.