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目的 研究HIV AIDS患者外周血CD4 + 、CD8+ 淋巴细胞数在不同条件下 (时间、温度和处理过程 )的变化。方法 选取HIV AIDS患者 34例 ,用流式细胞术检测在 4℃条件下放置不同时间(2、2 4、4 8、72h)的外周血CD4 + 、CD8+ 细胞数的变化 ,对经过处理的外周血 (处理过的血样 )CD4 + 、CD8+ 细胞数的变化进行比较 ;对室温条件下放置不同时间 (2、2 4、4 8、72h)处理过的血样CD4 + 、CD8+细胞数的变化进行比较。结果 在 4℃时 ,全血放置 2、2 4、4 8、72h的CD4 + 细胞计数差异无显著意义(P >0 0 5 ) ,而CD8+ 细胞数放置 72h时则差异有显著意义 (P <0 0 5 ) ;处理过的样品放置 72hCD4 +细胞计数差异才有显著意义 (P <0 0 5 ) ,而CD8+ 细胞数在 2 4h时差异就有显著意义 (P <0 0 5 )。在室温时 ,处理过血样放置 2、2 4、4 8、72h的CD4 + 细胞计数差异无显著意义 (P >0 0 5 ) ,而CD8+ 细胞数在 4 8h则差异有显著意义 (P <0 0 5 )。结论 抗凝全血在 4℃放置 4 8h ,检测CD4 + 、CD8+ 淋巴细胞数 ,结果是可靠的。处理过血样在室温放置 2 4h ,检测CD4 + 、CD8+ 淋巴细胞数 ,结果是可靠的。 2 4～4 8h虽然CD4 + 淋巴细胞没有变化 ,但CD8+ 淋巴细胞却发生明显的变化 ,两者比例必然发生变化。
Objective To study the changes of CD4 +, CD8 + lymphocytes in peripheral blood of HIV-infected patients under different conditions (time, temperature and treatment). Methods Totally 34 HIV / AIDS patients were selected. The changes of CD4 +, CD8 + cells in peripheral blood were detected by flow cytometry at 4 ℃ for different times (2,2,4,48,72h) Blood (treated blood samples) CD4 +, CD8 + cell number changes were compared; at room temperature for different time (2,2,4,48,72h) treated blood samples CD4 +, CD8 + cell number changes were compared . Results At 4 ℃, there was no significant difference in CD4 + cell counts between 2,24,88 and 72h in whole blood (P> 0.05), while the number of CD8 + cells was significant at 72h (P < (P <0.05). However, there was significant difference (P <0.05) in the difference of 72hCD4 + cell count between the treated and untreated samples. At room temperature, there was no significant difference in the counts of CD4 + cells treated with blood samples for 2,2,4,48,72h (P> 0 05), while the number of CD8 + cells was significant at 48h (P <0 0 5). Conclusion Anticoagulant whole blood was stored at 4 ℃ for 48 h and the numbers of CD4 + and CD8 + lymphocytes were determined. The results were reliable. Treated blood samples were placed at room temperature for 24 hours, the detection of CD4 +, CD8 + lymphocyte count, the result is reliable. 2 4 ~ 4 8h Although CD4 + lymphocytes did not change, but CD8 + lymphocytes have undergone significant changes, the ratio between the two inevitable changes.