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目的:以高浓度皮质酮(Corticosterone,CORT)损伤细胞制作原代培养的海马病理细胞模型,观察左归丸、右归丸对模型细胞的作用。方法:以新生SD乳鼠海马组织为材料,原代培养大鼠海马神经细胞;采用10-4mol/LCORT损伤细胞制作海马病理细胞模型,并用RU38486拮抗皮质酮的损伤作用。采用四甲基氮唑兰(MTT)法测定培养细胞的活性,RT-PCR法检测培养细胞皮质酮受体(GR)mRNA的表达变化,以及观察左归丸、右归丸对模型细胞上述各项指标变化的影响。结果:细胞的培养液中CORT浓度为10-4mol/L时,海马神经的细胞活性和GRmRNA表达均下降;左归丸、右归丸可以增强细胞活性和上调GRmRNA的表达。结论:浓度为10-4mol/L的CORT可以损伤细胞,制作原代培养的海马病理细胞模型;左归丸、右归丸对模型细胞的各项指标变化有明显的改善作用。
Objective: To observe the effect of Zuogui Pill and Yougui Pill on the model cells of primary cultured hippocampal cells by using high concentration corticosterone (CORT) injured cells. Methods: Hippocampal neurons were cultured in neonatal SD rats. Primary cultured rat hippocampal neurons were cultured in the medium of 10-4mol / L LCORT. The cells were treated with RU38486 for antagonizing the injury of corticosterone. The activity of cultured cells was determined by MTT assay. The expression of corticosterone receptor (GR) mRNA in cultured cells was detected by RT-PCR. The effects of Zuogui pill and Yougui pill The impact of changes in indicators. Results: The cell viability and the mRNA expression of GR mRNA in the hippocampal neurons were decreased when CORT concentration was 10-4mol / L in the culture medium. Zuoguiwan and Yougui Wan could increase the cell viability and up-regulate the expression of GR mRNA. CONCLUSION: CORT at the concentration of 10-4mol / L can damage cells and make the primary cultured hippocampal pathological cell model. Zuogui pill and Yougui pill can obviously improve the changes of the model cells.