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人单核-巨噬细胞系统在抗恶性疟原虫(P.f.)红内期无性体的保护性免疫中起重要作用,但由于血源不足及混杂淋巴细胞等因素的影响,不易获得实验所需的人单核-巨噬细胞。单核细胞系THP-1是原单核细胞中的非贴壁部分,作者用PHA刺激的人外周血单核细胞培养液培育THP-1细胞,使之分化成具有呼吸爆发、表达CR3、FcγR受体等特征的贴壁型THP-1单核细胞,然后研究它在一些调理素和细胞因子杀伤P.f.中的作用。
The human monocyte-macrophage system plays an important role in the protective immunity against the clonogenicity of Plasmodium falciparum (Pf), but due to the lack of blood supply and the influence of mixed lymphocytes, it is not easy to obtain the required experimental Human monocytes - macrophages. The monocyte cell line THP-1, a non-adherent part of the original monocytic cells, was cultured in PHA-stimulated human peripheral blood mononuclear cell cultures to differentiate into THP-1 cells with respiratory burst, expression of CR3, FcγR receptors And other characteristics of adherent THP-1 mononuclear cells, and then investigate its role in some of the opsins and cytokines Pf.