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目的探讨瘦素(leptin)及其受体(Ob-Ra、Ob-Rb)与食管鳞癌的关系,为食管鳞癌的发病机制研究提供一定的理论依据。方法 RT-PCR法检测20例正常食管和24例食管鳞癌组织中leptin、Ob-Ra、Ob-Rb的mRNA表达。结果 (1)20例正常食管组织中leptin、Ob-Ra、Ob-Rb的mRNA表达阳性率分别为50.0%、50.0%、40.0%;24例食管鳞癌组织中leptin、Ob-Ra、Ob-Rb的mRNA表达阳性率分别为79.2%、83.3%、83.3%。正常食管组与食管鳞癌组相比差异均有统计学意义(P<0.05)。(2)食管鳞癌组织中leptin、Ob-Ra、Ob-Rb的mRNA相对表达量亦高于正常食管组织,差异均有统计学意义(P<0.05)。结论瘦素及其受体在食管鳞癌组织中的表达阳性率和相对表达量均高于正常食管组织,提示瘦素及其受体可能在食管鳞癌的发生发展中发挥重要作用。
Objective To investigate the relationship between leptin and its receptor (Ob-Ra, Ob-Rb) and esophageal squamous cell carcinoma (ESCC), and to provide a theoretical basis for the study on the pathogenesis of esophageal squamous cell carcinoma. Methods The mRNA expressions of leptin, Ob-Ra and Ob-Rb in 20 normal esophagus and 24 esophageal squamous cell carcinoma tissues were detected by RT-PCR. Results (1) The positive rates of leptin, Ob-Ra and Ob-Rb mRNA in 20 normal esophageal tissues were 50.0%, 50.0% and 40.0% The positive rate of Rb mRNA expression was 79.2%, 83.3% and 83.3% respectively. The difference between normal esophageal squamous cell carcinoma and esophageal squamous cell carcinoma was statistically significant (P <0.05). (2) The relative mRNA expression of leptin, Ob-Ra and Ob-Rb in esophageal squamous cell carcinoma was also higher than that in normal esophageal tissue (P <0.05). Conclusion The positive rate and relative expression of leptin and their receptors in esophageal squamous cell carcinoma tissues are higher than those in normal esophageal tissues, suggesting that leptin and its receptors may play an important role in the development of esophageal squamous cell carcinoma.