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目的初步鉴定人胚肺成纤维细胞(HL)突变株Z-HL16C的转化性质。方法采用细胞形态学观察、细胞遗传学染色体分析、细胞在裸鼠体内恶性生长情况的组织病理学检测以及细胞对病毒的敏感性等方法。结果HL细胞转化的Z-HL16C细胞在形态上由梭形的纤维细胞变成多边形的上皮样细胞,且具有稳定无限传代等特点;染色体数目由正常人的46条二倍体,转化成多数为亚三倍体或超三倍体;染色体结构核型中约有30%~40%的染色体为结构异常染色体;裸鼠移植肿瘤发病率为100%,其瘤组织病理检测结果显示,该细胞株为癌变细胞株。病毒实验表明,Z-HL16C细胞对单纯疱疹等多种常见病毒高度敏感。结论人胚肺成纤维细胞的突变株Z-HL16C是对病毒高度敏感的癌细胞株。
Objective To initially identify the transforming properties of human embryonic lung fibroblasts (HL) mutant Z-HL16C. Methods Cell morphological observation, cytogenetic analysis of chromosomes, cell malignant growth in nude mice, histopathology and cell sensitivity to the virus and other methods. Results HL-HL16C cells transformed from spindle-shaped fibroblasts into polygonal epithelial-like cells with stable and infinitely passaged passages. The number of chromosomes was changed from 46 diploids of normal individuals to majority Sub-triploid or triploid; chromosomal karyotype about 30% to 40% of chromosomes for structural abnormalities chromosome; nude mice transplantation tumor incidence was 100%, the tumor histopathological examination showed that the cell line For cancer cell lines. Virus experiments show that Z-HL16C cells are highly susceptible to many common viruses such as herpes simplex. Conclusion The mutant strain Z-HL16C of human embryo lung fibroblasts is a highly virus-sensitive cancer cell line.