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目的观察早期补充不同剂量维生素D对哮喘大鼠肺组织CC族趋化因子嗜酸粒细胞激活趋化因子(eotaxin)和受体CCR3表达的影响。方法对用卵清蛋白(OVA)建立的大鼠哮喘模型进行1,25-(OH)2VitD3干预,分为低、中、高三个剂量,采用细胞计数、免疫组织化学染色和Westernblot方法检测哮喘大鼠肺组织炎症和eotaxin及CCR3的变化。结低中剂量维生素D可以减轻哮喘的炎性浸润,表现为肺泡灌洗液(BALF)中白细胞总数减少,嗜酸粒细胞(EOS)百分比下降,eotaxin和CCR3蛋白质表达减弱。相反,高剂量维生素D则促进哮喘的发作。结论早期补充低中剂量的维生素D对哮喘大鼠肺组织中eotaxin和CCR3有抑制作用,而高剂量维生素D则会促进eotaxin和CCR3的表达,从而加剧大鼠哮喘的气道炎症。
Objective To observe the effects of early supplementation with different doses of vitamin D on CC chemokines eotaxin and CCR3 expression in lung tissue of asthmatic rats. Methods Rat asthma model established by ovalbumin (OVA) was subjected to 1,25- (OH) 2VitD3 intervention, divided into low dose, middle dose and high dose. The cell count, immunohistochemical staining and Western blot were used to detect large asthma Lung tissue inflammation and eotaxin and CCR3 changes. Results Low and medium doses of vitamin D attenuated the inflammatory infiltrate of asthmatics by reducing the total number of leukocytes, decreasing the percentage of eosinophils (EOS), and decreasing the expression of eotaxin and CCR3 in BALF. In contrast, high doses of vitamin D promote asthma attacks. Conclusion Early low and middle doses of vitamin D can inhibit eotaxin and CCR3 in the lung tissue of asthmatic rats, whereas high doses of vitamin D can promote the expression of eotaxin and CCR3, thereby exacerbating airway inflammation in asthmatic rats.