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乳腺癌是女性肿瘤中发病率最高,且致死率仅次于肺癌的癌症种类。大部分乳腺癌病人死亡的主要原因是癌细胞向远端器官发生了转移。在本文中,我们研究了IL-13Rα2与乳腺癌转移以及病人不良预后的相关性,并初步探寻了其影响癌细胞转移的可能机制。通过实时荧光定量PCR、蛋白免疫印迹实验、Kaplan-Meier生存分析、细胞免疫荧光和细胞侵袭等实验方法,我们证明了IL-13Rα2的表达与ER阴性乳腺癌细胞的肺转移能力、ER阴性乳腺癌病人的肿瘤转移和不良预后正相关,与乳腺癌样本中ER的表达负相关,并且其中的作用机制可能与IL-13通过IL-13Rα2调节肿瘤细胞上皮-间质转换(EMT)有关。
Breast cancer is the highest incidence of cancer in women, and the second leading cause of death in cancer of lung cancer types. Most of the major causes of death of breast cancer patients is the cancer cells to the distal organ metastasis. In this paper, we investigated the correlation between IL-13Rα2 and breast cancer metastasis and poor prognosis in patients, and explored its possible mechanism of affecting cancer cell metastasis. We demonstrated that IL-13Rα2 expression is associated with lung metastasis of ER-negative breast cancer cells, ER-negative breast cancer by real-time fluorescence quantitative PCR, Western blotting, Kaplan-Meier survival analysis, immunofluorescence and cell invasion assays The patient’s tumor metastasis is positively correlated with poor prognosis, and negatively correlated with the expression of ER in breast cancer samples, and the mechanism may be related to IL-13 regulating tumor cell epithelial-mesenchymal transition (EMT) through IL-13Rα2.