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目的观察脱氢表雄酮对大鼠大脑皮质神经元谷氨酸释放的影响。方法原代培养的SD大鼠大脑皮质神经元经不同浓度(1×10-7、1×10-6、1×10-5mol·L-1)和不同作用时间(0.5,1,1.5,2,5,24,36,48,72h)的脱氢表雄酮处理后,采用OPA-巯基乙醇柱前衍生,荧光检测-高效液相色谱法测定细胞培养液中谷氨酸的含量。结果与对照组相比,不同浓度的脱氢表雄酮处理后,细胞培养液中谷氨酸的水平均下降(P<0.05或P<0.01);与对照组相比,脱氢表雄酮处理组1,1.5,2,5,24,36,48,72h细胞培养液中谷氨酸的水平均下降(P<0.05或P<0.01)。结论脱氢表雄酮可抑制神经元谷氨酸的释放,这可能介导了其对抗谷氨酸兴奋性毒性的作用。
Objective To observe the effect of dehydroepiandrosterone on glutamate release from rat cortical neurons. Methods Primary cultured rat cerebral cortical neurons were cultured in different concentrations (1 × 10-7, 1 × 10-6, 1 × 10-5mol·L-1) and different time (0.5,1,1.5,2 , 5,24,36,48,72h) dehydroepiandrosterone treatment, using OPA-mercaptoethanol pre-column derivatization, fluorescence detection - high performance liquid chromatography determination of cell culture medium glutamic acid content. Results Compared with the control group, the levels of glutamic acid in the cell culture medium decreased after treatment with different concentrations of dehydroepiandrosterone (P <0.05 or P <0.01). Compared with the control group, dehydroepiandrosterone treatment The levels of glutamate in the cell culture medium of group 1, 1.5, 2, 5, 24, 36, 48 and 72h decreased (P <0.05 or P <0.01). Conclusion Dehydroepiandrosterone can inhibit the release of glutamate in neurons, which may mediate its anti-glutamate excitotoxicity.