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为了解土源性线虫传播途径和流行因素,我们于1999年7～9月对土源性线虫污染蔬菜的情况进行了调查。1 材料和方法1.1 标本采样:在济南市五处菜市场随机采取新鲜蔬菜,每份约500g;在五家超市随机采取“净菜”标本,每份约250g。1.2 标本的清洗和浓缩:用毛刷蘸取自来水刷洗蔬菜标本,“净菜”用自来水直接浸洗,将洗过的水倒入锥形杯中,待其自然沉淀。1h后倾去其上层液,再放入清水,搅拌后静置。如此反复水洗沉淀,直至上液清晰为止。倾去上液,每份留
In order to understand the routes and epidemic factors of soil-borne nematodes, we investigated the contamination of vegetables by soil-borne nematodes from July to September 1999. 1 Materials and methods 1.1 Sample Sampling: Randomly take fresh vegetables in five vegetable markets in Jinan City, each about 500g; in five supermarkets to take “clean dishes” specimens, each about 250g. 1.2 Specimen cleaning and concentration: Brush with a brush dipped in water scrub vegetable specimens, “clean dishes” directly dipped in tap water, the washed water into the conical cup until it is natural precipitation. 1h after pouring the upper liquid, then add water, stirring and let stand. So repeatedly washed sediment, until the liquid clear so far. Pour on the liquid, each stay