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目的探讨日本血吸虫中国大陆株２６ｋＤａ基因重组蛋白对宿主肝虫卵肉芽肿形成的影响。方法将 ＩＣＲ雄性小鼠分成 ２组，１组经 ｒＳｊｃ２６ＧＳＴ免疫，另 １组为对照。免疫结束后第 ５天，２组小鼠均感染日本血吸虫尾蚴 ４０条±１条／鼠，于 ６周后剖杀，取肝组织进行观察。结果免疫组与对照组肝表面虫卵结节数分别为５．６９±１．１９和２０．４７±１．８３，前者比后者减少７２．２０％；肝肉芽肿平均直径免疫组为 １４０．００ μｍ±３８．２４ μｍ，与对照组２４５．７２ μｍ±３２．７３ μｍ相比，减少４３．０２％；同时免疫组小鼠体内特异性抗ｒＳｊｃ２６ＧＳＴ抗体水平明显高于对照组。结论ｒＳｊｃ２６ＧＳＴ对日本血吸虫具有明显的抗病效果。
Objective To investigate the effect of 26kDa recombinant protein of Schistosoma japonicum on the formation of host hepatic granuloma in Henan Province. Methods ICR male mice were divided into two groups, one was immunized with rSjc26GST and the other was control. On the 5th day after the immunization, both groups of mice were infected with 40 ± 1 mouse / worm of cercariae of Schistosoma japonicum, and were killed after 6 weeks. Liver tissues were taken for observation. Results The number of eggs on the surface of the liver in the immunized group and the control group were 5.69 ± 1.19 and 20.47 ± 1.83, respectively. The former was reduced by 72.20% compared with the latter. The mean diameter of the granuloma was 140 .00 μm ± 38.24 μm, which was reduced by 43.02% compared with the control group 245.72 μm ± 32.73 μm. The specific anti-rSjc26GST antibody level in the immunized group was significantly higher than that in the control group. Conclusion rSjc26GST has significant resistance to Schistosoma japonicum.