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目的观察黄精多糖(PSP)对糖尿病心肌病大鼠心肌纤维化影响,探讨其可能机制。方法健康雄性SD大鼠,随机分为对照组、模型组、黄精多糖250、500、1000 mg/kg组,所有大鼠给予普通饲料,连续12周,观察各组大鼠一般情况,检测空腹血糖,血生化指标,血流动力学指标,计算心脏质量指数(HMI),左心室质量指数(LVMI),苏木素-伊红染色观察心肌形态学变化;Masson染色观察心肌胶原纤维分布;免疫组化法检测心肌组织骨形态发生蛋白-7(BMP-7)、转化生长因子β1(TGF-β1)、Smad2/3、Smad7蛋白表达。结果与对照组比较,模型组大鼠空腹血糖[(24.81±2.30)mmol/L]、HMI[(4.16±1.14)]、LVMI[(2.85±0.09)]及LVEDP升高(P<0.05);免疫组化结果显示,模型组大鼠心肌组织中TGF-β1、Smad2/3蛋白的表达显著增加(P<0.05),BMP-7、Smad7蛋白表达明显减少(P<0.05);HE、M asson染色显示模型组大鼠心肌组织病理损伤严重。与模型组比较,黄精多糖1 000 mg/kg组大鼠空腹血糖[(13.28±2.08)mmol/L]、HMI[(3.21±0.07)]、LVMI[(2.09±0.02)]及LVEDP下降(P<0.05),心肌组织中TGF-β1、Smad2/3蛋白表达显著减少(P<0.05),BM P-7、Smad7蛋白表达明显升高(P<0.05);心肌组织病理改变有所改善。结论黄精多糖可改善糖尿病大鼠心肌纤维化,其机制可能与促进心肌组织中BMP-7表达,影响TGF-β1/Smads信号通路有关。
Objective To observe the effect of Polygonatum Polysaccharide (PSP) on myocardial fibrosis in diabetic cardiomyopathy rats and to explore its possible mechanism. Methods Healthy male Sprague-Dawley rats were randomly divided into control group, model group, Polygonatum polysaccharide 250,500,1000 mg / kg group, all rats were given normal feed for 12 weeks, the general situation of rats in each group were observed, fasting blood glucose , Blood biochemical parameters, hemodynamic parameters, cardiac function index (HMI), left ventricular mass index (LVMI) and myocardial morphology were observed by hematoxylin-eosin staining. Masson staining was used to observe myocardial collagen distribution. Immunohistochemistry The expression of BMP-7, TGF-β1, Smad2 / 3 and Smad7 in myocardium were detected. Results Compared with the control group, the fasting blood glucose ([(24.81 ± 2.30) mmol / L], the HMI [(4.16 ± 1.14)], the LVMI (2.85 ± 0.09) and the LVEDP in the model group were significantly increased The results of immunohistochemistry showed that the expression of TGF-β1 and Smad2 / 3 in model group was significantly increased (P <0.05), while the expression of BMP-7 and Smad7 in model group was significantly decreased (P <0.05); HE and M asson Staining showed that myocardial injury in model group was serious. Compared with model group, fasting plasma glucose ([(13.28 ± 2.08) mmol / L], HMI [(3.21 ± 0.07)], LVMI [(2.09 ± 0.02)] and LVEDP decreased (P <0.05). The expression of TGF-β1 and Smad2 / 3 in myocardium was significantly decreased (P <0.05), and the expressions of BM-P-7 and Smad7 in myocardium were significantly increased (P <0.05). Conclusion Polygonatum Polysaccharide can improve myocardial fibrosis in diabetic rats. The mechanism may be related to the promotion of BMP-7 expression in myocardium and the influence of TGF-β1 / Smads signaling pathway.