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应用逆转录病毒载体介导基因转移法将ｎｅｏ~ｒ基因导入人造血干祖细胞，并经体外液体长期培养。结果表明，产重祖病毒细胞ＰＡ３１７／Ｎ２的病毒滴度最高达６．５×１０~５ＣＦＵ／ｍｌ，用ＰＣＲ法从体外培养２、４、６ｗ及加入ｒＩＬ－１、ｒＩＬ－６的悬浮和贴壁的转染细胞中均可扩增出ｎｅｏ~ｒ基因ｃＤ－ＮＡ片段，非同位素化学发光法标记ｎｅｏ~ｒ探针与之杂交显示阳性结果。表明逆转录病毒介导目的基因已有效地转入体外培养的人造血干祖细胞中，并进行表达。
The neo ~ r gene was introduced into human hematopoietic stem / progenitor cells by retroviral vector-mediated gene transfer and cultured in long-term liquid in vitro. The results showed that the virus titer of PA317 / N2 was up to 6.5 × 10 ~ 5CFU / ml. The PCR products were cultured in vitro for 2, 4, 6 weeks, rIL-1, rIL-6 and The cD-NA fragment of neo ~ r gene could be amplified in adherent cells. The hybridization of neo ~ r probe with non-isotope chemiluminescence method showed positive results. This indicated that the retrovirus-mediated gene has been efficiently transferred into human hematopoietic stem and progenitor cells cultured in vitro and expressed.