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[目的]对SGTA基因及其蛋白的结构和特征进行生物信息学分析,为研究SGTA与肿瘤形成和发展的相关性提供理论基础。[方法]运用生物信息学数据库和软件对SGTA基因的结构、单核苷酸多态性位点(SNP)、SGTA基因与其他基因的相互作用网络、SGTA蛋白的理化性质、二级结构、蛋白结构域、蛋白翻译后修饰、蛋白质之间相互作用网络进行分析。[结果]人SGTA基因有5种可变剪接产物,编码区存在78个SNP位点,其中错义突变31个,无义突变1个。人SGTA蛋白由313个氨基酸组成,是稳定性不高的亲水蛋白,α-螺旋是其主要二级结构元件,属于TRP超家族,预测有3个磷酸化激酶修饰位点和数个潜在泛素化修饰位点。与SGTA存在相互作用的基因和蛋白多数与维持体内蛋白质稳定的分子伴侣功能相关。[结论]SGTA基因及其蛋白的生物信息学分析为进一步实验研究其在肿瘤形成和发展中的地位及调控机制奠定了基础。
[Objective] The bioinformatics analysis of the structure and characteristics of SGTA gene and its proteins provided the theoretical basis for studying the relationship between SGTA and tumor formation and development. [Method] The structure of SGTA gene, single nucleotide polymorphism site (SNP), the interaction network of SGTA gene and other genes, the physicochemical properties of SGTA protein, secondary structure, protein Domain, protein post-translational modification, protein-protein interaction networks. [Result] There were five kinds of alternative splicing products of human SGTA gene. There were 78 SNPs in the coding region, including 31 missense mutations and 1 nonsense mutation. The human SGTA protein consists of 313 amino acids and is a poorly-stable hydrophilic protein. Alpha-helix is a major secondary structural element of TRP and belongs to the TRP superfamily. Three phosphorylation kinase sites and several potential pan- Amino acid modification sites. The majority of genes and proteins that interact with SGTA are associated with the maintenance of protein-stable molecular chaperone function in vivo. [Conclusion] The bioinformatics analysis of SGTA gene and its protein laid the foundation for the further experimental study of its role in tumor formation and development and its regulatory mechanism.