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应用巢式-PCR方法,对深圳检出的2份HIV-1感染者的外周血单核细胞样本进行扩增,获得HIV-1膜蛋白(env)基因核酸片段,并对其C_2-V_3区及邻区350～450个核苷酸序列进行测定和分析.结果表明这2份血样为HIV-1B亚型毒株感染(sz-B),彼此间的基因离散率为10.2%;与A-E国际参考亚型序列比较,sz-B与A及C-E参考亚型共享序列间的基因离散率均大于22%,而与欧美B亚型(Boon)间的基因离散率仅为7.5%;与国内部分地区流行的B亚型代表株比较其基因离散率为11%～12%.提示HIV-1B亚型在深圳的流行时间约为10年,主要经性途径由境外传入,且经性传播途径在人群中流行.
Using nested-PCR method, peripheral blood mononuclear cells from 2 HIV-1 infected patients in Shenzhen were amplified to obtain HIV-1 membrane protein (env) gene nucleic acid fragment, and its C_2-V_3 region And 350 ~ 450 nucleotide sequences in the adjacent area were measured and analyzed.The results showed that the two blood samples were infected with HIV-1B subtype (sz-B) with a genetic divergence rate of 10.2% According to the comparison of subtype sequences, the gene dispersal rates between sz-B and A and CE reference subtypes were both greater than 22% and only about 7.5% to those between European and American subtype Boon. The prevalence of representative strains of subtype B is 11% ~ 12% compared with that of the representative strains of subtype B. This indicates that the prevalence of HIV-1B subtype is about 10 years in Shenzhen, mainly through the sexual route from outside the country, Popular in the crowd.