论文部分内容阅读
目的调查舒巴坦敏感β-内酰胺酶(β-lactamase)的本底含量和添加状况,以期了解甘肃地区生鲜乳品的本底和添加水平。方法采集甘肃省牛奶养殖场(户)、乳品公司生产的乳头奶、原料奶、成品奶各50份样品,用杯碟法对舒巴坦敏感β-内酰胺酶进行定性和半定量分析。结果β-内酰胺酶检出率乳头奶为2.0%,原料奶为20.0%,成品乳为6.0%;其中奶厂1和品牌1为国家知名品牌,其原料奶β-内酰胺酶检出率为5.3%,奶厂2和品牌2、奶厂3和品牌3为省内品牌,其原料奶β-内酰胺酶检出率分别为23.5%及35.7%;品牌1与品牌2和品牌3内部管理和检测手段存在一定的差异,原料奶中β-内酰胺酶检出率也存在明显的差异。结论奶牛养殖户在原料乳和成品乳中属人为添加β-内酰胺酶,应该引起足够的重视;今后要加强奶源的管理,进一步做好牛奶的安全监控工作。
Objective To investigate the background content and addition status of sulbactam-sensitive β-lactamase in order to understand the background and the level of fresh dairy products in Gansu Province. Methods Fifty samples of nipple milk, raw milk and finished milk from milk farms (households) and dairy companies in Gansu Province were collected. Qualitative and semi-quantitative analysis of sulbactam-sensitive β-lactamases were performed by cup-saucer method. Results The detection rate of β-lactamase was 2.0% for nipple milk, 20.0% for raw milk and 6.0% for finished milk. Among them, milk factory 1 and brand 1 were well-known national brands. The detection rate of β-lactamase in raw milk 5.3%, milk factory 2 and brand 2, milk factory 3 and brand 3 are provincial brands, the detection rates of their raw milk β-lactamase were 23.5% and 35.7% respectively; the brands 1 and 2 and 3 Management and testing methods there are some differences in the raw milk β-lactamase detection rate there are significant differences. Conclusion Dairy farmers in the raw milk and finished milk is an artificially added β-lactamase, should pay enough attention; in the future to strengthen the management of milk, to further improve the safety of milk monitoring.