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本文利用ＰＣＲ技术建立一种对ＨＳＶ直接基因分型的方法。在ＨＳＶ－Ⅰ、Ⅱ两型的ＤＮＡ多聚酶基因上设计了一条两型共同的上游引物（ＨＤＰ－Ｂ）和两条型特异的下游引物（ＨＤＰ－１、ＨＤＰ－２）。三条引物共同组成一个扩增反应体系，在ＨＳＶ－Ⅰ产生５４３ｂｐ条带，ＨＳＶ－Ⅱ产生３７２ｂｐ条带，据此在基因水平上对ＨＳＶ进行分型。５株不同来源的ＨＳＶ（２株Ⅰ型，３株Ⅱ型）分型结果与病毒分离及血清学方法完全一致。该反应体系与其它来源的ＤＮＡ不产生特异反应，敏感性可达１ｆｇ。应用该法对１５１份临床可疑ＨＳＶ感染的标本进行检测并分型，结果与免疫学方法完全一致。
This article uses PCR techniques to establish a direct HSV genotyping method. A two-type common upstream primer (HDP-B) and two type-specific downstream primers (HDP-1, HDP-2) were designed on the HSV-Ⅰ and Ⅱ DNA polymerase genes. The three primers together form an amplification reaction system, producing 543bp band in HSV-I and 372bp band in HSV-II, thereby typing HSV at the gene level. The typing results of 5 strains of HSV (2 strains of type I and 3 strains of type II) were completely consistent with those of virus isolation and serological methods. The reaction system and other sources of DNA does not produce specific reactions, the sensitivity of up to 1fg. The method was used to detect and classify 151 specimens of clinically suspected HSV infection. The results were in good agreement with immunological methods.