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目的探讨一氧化氮合酶(NOS)、神经肽Y(NPY)和胎盘凋亡细胞在妊娠期肝内胆汁淤积(ICP)发生发展中的作用。方法随机选取33例ICP患者为研究组,35例正常妊娠孕妇为对照组,采用免疫组织化学技术(IH)检测两组胎盘组织中NOS、NPY的表达情况;采用酶联免疫吸附法(ELISA)检测两组母体血浆NOS、NPY的表达水平;采用Tunnel染色技术和测定线粒体活性氧检测胎盘细胞的凋亡情况。结果 NOS在研究组胎盘的表达明显低于对照组,差异具有统计学意义(P﹤0.05);NPY在研究组胎盘的表达明显高于对照组,差异具有统计学意义(P﹤0.05);NOS和NPY在两组血浆中均有表达,NOS在研究组的血浆表达明显低于对照组,差异具有统计学意义(P﹤0.05),NPY在研究组的血浆表达明显高于对照组,差异具有统计学意义(P﹤0.05);研究组胎盘细胞的AI明显高于对照组,差异具有统计学意义(P﹤0.05);研究组胎盘组织线粒体的荧光强度达明显高于对照组,差异具有统计学意义(P﹤0.05)。结论 NOS、NPY的表达异常和胎盘细胞凋亡可能与ICP的发病、胎儿宫内窘迫和胎死宫内有关。
Objective To investigate the role of nitric oxide synthase (NOS), neuropeptide Y (NPY) and placental apoptotic cells in the development of intrahepatic cholestasis of pregnancy (ICP). Methods Totally 33 ICP patients were selected as research group and 35 normal pregnant women as control group. Immunohistochemistry (IH) was used to detect the expression of NOS and NPY in placentas of both groups. Enzyme-linked immunosorbent assay (ELISA) The plasma levels of NOS and NPY in the two groups were detected. The apoptosis of placental cells was detected by Tunnel staining and mitochondrial reactive oxygen species (ROS) detection. Results The expression of NOS in the study group was significantly lower than that in the control group (P <0.05). The expression of NPY in the study group was significantly higher than that in the control group (P <0.05) (P <0.05). The plasma levels of NPY in the study group were significantly higher than those in the control group, the differences were statistically significant (P <0.05). The AI of placental cells in the study group was significantly higher than that of the control group (P <0.05). The fluorescence intensity of placental mitochondria in the study group was significantly higher than that in the control group Significance (P <0.05). Conclusion The abnormal expression of NOS and NPY and the apoptosis of placental cells may be related to the incidence of ICP, fetal distress and intrauterine fetal death.