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目的 观察不同类型垂体腺瘤患者血清ＩＬ－６、ＴＮＦ－α水平，ＮＫ细胞活性和淋巴细胞亚型的变化。方法 根据垂体激素水平将３２例垂体腺瘤患者分为非分泌性垂体腺瘤和分泌性垂体腺瘤两组。用酶联免疫吸附法检测血清ＩＬ－６、ＴＮＦ－α浓度，３Ｈ－ＴｄＲ掺入法检测ＮＫ细胞活性，ＡＰＡＡＰ桥联酶标法进行淋巴细胞亚群分型。结果 各种类型垂体腺瘤血清ＩＬ－６、ＴＮＦ－α水平无明显变化，分泌催乳素（ＰＲＬ）腺瘤患者ＣＤ３＋ 、ＣＤ４＋ 细胞亚群比例明显低于正常对照组（Ｐ＜ ０．０５），分泌ＡＣＴＨ腺瘤患者ＮＫ细胞活性降低，Ｔ４细胞亚群减少，而非分泌性腺瘤患者细胞免疫功能无明显异常。结论 垂体腺瘤患者内分泌异常可干扰其细胞免疫功能。
Objective To observe the changes of serum IL-6, TNF-α levels, NK cell activity and lymphocyte subsets in different types of pituitary adenomas. Methods According to pituitary hormone levels, 32 patients with pituitary adenomas were divided into two groups: nonsecreting pituitary adenomas and secretory pituitary adenomas. Serum IL-6 and TNF-α levels were measured by enzyme-linked immunosorbent assay, NK cell activity was measured by 3H-TdR incorporation, and lymphocyte subpopulations were profiled by APAAP bridging enzyme method. Results The serum levels of IL-6 and TNF-α in all types of pituitary adenomas had no significant changes. The proportion of CD3+ and CD4+ subpopulations in prolactin-secreting adenomas was significantly lower than that in normal controls (P<0.05). The activity of NK cells in patients with ACTH-secreting adenomas was decreased, and T4 cell subsets were decreased. There was no significant abnormality in cellular immune function in patients with nonsecreting adenomas. Conclusion Endocrine abnormalities in patients with pituitary adenoma can interfere with its cellular immune function.