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以百日草种子为试材,通过生物统计和分子标记的方法,对航天搭载处理的百日草干种子当代植株生长状况进行了研究。结果表明:航天搭载处理使百日草种子的发芽率有所下降;而航天搭载处理组的百日草当代幼苗黑斑病的感染率有一定升高;在航天搭载处理组的变异植株中发现1株稳定的三叶共生变异株H1-2,变异株的叶片明显小于对照植株的叶片。随机扩增多态性DNA(RAPD)分析表明,与对照植株相比H1-2基因组的变异率为13.4%。航天搭载诱导百日草生物学效应的研究为花卉的航天诱变育种提供了一定的参考依据。
Taking the Zinnia elegans seeds as test materials, the growth status of Zinnia sativa seeds treated by space shuttle was studied by means of biometrics and molecular markers. The results showed that the germination rate of Zinnia prunella seeds was decreased with space-borne treatment, while the infection rate of Z. japonica seedling black spot disease was increased in space-borne treatment group. In the mutant plants with space-borne treatment group, One stable clover symbiotic variant H1-2, mutant leaves was significantly smaller than the control plant leaves. Random amplified polymorphic DNA (RAPD) analysis showed that the variation rate of H1-2 genome was 13.4% compared with the control plants. Space shuttle carrying zinnia induced biological effects of the study of space-induced mutation breeding flowers provide some reference.