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目的 探讨廿二碳六烯酸 (DHA)影响阿霉素 (ADM)对人乳腺癌DMA MB 435s细胞毒活性的机理是否通过DHA的脂质过氧化作用。方法 在培养的人乳腺癌DMA MB 435s细胞株中加入不同配伍的细胞毒药物、多不饱和脂肪酸 (PUFAs)、前氧化剂VitC和VitK3混合物及抗氧化剂VitE等 ,在细胞的抽提物中以TBA法每 2 4小时测定脂质过氧化产物丙二醛 (MDA)及用亚硝酸盐分光光度法测定一氧化氮 (NO)的含量 ,并作出MDA、NO含量与细胞毒性间的剂量 -效应相关直线。结果 从细胞培养的第三天开始 ,出现明显的细胞活力变化 ,同时伴有脂质过氧化物 (MDA)水平的增加及NO含量的降低。细胞抽提物中MDA及NO含量与细胞毒性之间存在直线相关关系。结论 DHA能明显增加ADM对MDA MB 435s细胞株的细胞毒活性 ,机理之一是DHA增强了瘤细胞内的脂质过氧化作用。
Objective To investigate whether the mechanism by which docosahexaenoic acid (DHA) affects the cytotoxic activity of doxorubicin (ADM) on human breast cancer DMA MB 435s cells is via lipid peroxidation of DHA. Methods Different proportions of cytotoxic drugs, polyunsaturated fatty acids (PUFAs), VitC and VitK3 mixture and VitE were added into the cultured human breast cancer DMA MB 435s cell line. TBA Method was used to measure the content of malondialdehyde (MDA) and the content of nitric oxide (NO) by the method of nitrite spectrophotometry every 2-4 hours, and to make dose-effect correlation between MDA and NO content and cytotoxicity straight line. Results From the third day of cell culture, significant changes in cell viability accompanied by an increase in lipid peroxidation (MDA) and a decrease in NO content were observed. There was a linear correlation between MDA and NO content in cell extracts and cytotoxicity. Conclusion DHA can significantly increase the cytotoxic activity of ADM on MDA-MB-435s cells. One of the mechanisms is that DHA enhances lipid peroxidation in tumor cells.