论文部分内容阅读
通过人工隔离诱导实现新疆早实核桃的孤雌生殖,孤雌生殖频率达8.17%～69.44%,且播种后孤雌生殖核桃出苗率可达5.26%～80%。研究过程中发现的品种核桃天然孤雌生殖植株,通过人工套袋隔离促其孤雌生殖,获得2株生长正常的孤雌生殖核桃播种苗。采用RAPD技术对孤雌品种进行鉴定,共用55条引物对其4个材料(914母本、914孤雌、914单株-1、914单株-2)进行扩增,结果表明这四个材料均无差异带出现,说明所有供试材料在基因组的大约450多个位点扩增的DNA片段没有差异,证明914孤雌单株与其母本无显著差异、为纯化基因型植株。利用孤雌生殖开展核桃优良品种遗传纯化,可最大限度地缩短纯化周期,为林木遗传纯化开拓一条快捷、高效、实用的新途径。
Parthenogenesis of parthenocarpic walnut in Xinjiang was induced by artificial isolation, the frequency of parthenogenesis was 8.17% -69.44%, and the emergence rate of parthenogenetic walnut was 5.26% -80% after sowing. The walnut natural parthenogenesis plants found in the research process were isolated and isolated by artificial bagging to promote parthenogenesis, and two normal growth parthenogenetic walnut seedlings were obtained. Parthenogenetic cultivars were identified by RAPD technique. A total of 55 primers were used to amplify four materials (914 maternal, 914 parthenogenetic, 914 single-1, 914 single-2). The results showed that the four materials No difference bands appeared, indicating that all tested materials in the genome of about 450 sites amplified DNA fragments showed no difference, that 914 parthenogenetic individuals and their maternal no significant difference for the purification of genotype plants. The use of parthenogenesis to carry out genetic purification of fine varieties of walnut, can minimize the purification cycle, for forest genetic purification to develop a fast, efficient and practical new way.